Valencia’s interim coach Varo took charge for an eighth time on a temporary basis after Gennaro Gattuso left by mutual consent on Monday, with the visiting defending champions having a tough night.
Los Che are in 14th place, just one point off the relegation zone, and will now be without experienced defender Gabriel.
“We had a good first half, that was our plan, leaving them a little bit of space, but in the second it was difficult for us,” Valencia’s Hugo Gilmón told DAZN.
Ancelotti again started at left-back with Eduardo Camavinga and made two changes to Luka Modric and Asensio from Sunday’s draw with Real Sociedad.